The experiment was performed on a confocal system. Searching mosaic was acquired with a 10x Dry objective covering the entire slide with a paraffin section of colon from mice. Object detection was customized for tissue pieces excluding by size single cells and irrelevant tissue pieces. NoIR function was enabled avoiding the capture of all the non informative fields surrounding the main piece of tissue. High resolution acquisition was done with a 20x OIL objective. After the capture, single fields were stitched