PDMS chips with 80 microns diameter nano-wells were placed on a glass bottom 24 well plate. Cells expressing different fluorescent proteins were seeded and different treatments were added to each chip. Searching mosaic covered the whole chip with a two channels acquisition (shown in red and green). Red searching mosaic shown was intended to identify nano-wells. Green searching mosaic was used to detect all green fluorescent cells. Final capture time lapse acquisition at high resolution (63x glycerol Oil, objective, 37ºC and 5%CO2), exclusively on the nano wells containing a single cell expressing the marker of interest.