The experiment was performed on a wide field system. Searching mosaic was acquired with a 10x Dry objective covering an entire slide with a Tissue Micro Array (pieces of tissue collection from different samples). Object detection was customized for every piece of tissue excluding single cells and irrelevant tissue pieces.. High resolution capture of 77 tissue pieces was automatically performed overnight with a 40x OIL objective. A variable size images mosaic was auto-adjusted to cover each piece of tissue (up to 6x6, 5 sections Z stacks, 3 channels)